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糧食、油料檢驗粗蛋白質測定法

ZHONGHUARENMINGONGHEGUOGUOJIABIAOZHUN

LIANGSHI、YOULIAOJIANYAN

CUDANBAIZHICEDINGFA

Inspection of grain and oilseeds

Methods for determination of crude protein

UDC (633.1+633.85).001.4

GB 5511-85


    BENBIAOZHUNSHIYONGYUSHANGPINLIANGSHI、YOULIAOZHONGCUDANBAIZHIHANLIANGDECEDING。

1  YIQIHEYONGJU

1.1  KAISHISHAOPING:50ml;

1.2  YUANDISHAOPING:1000ml;

1.3  萬用電爐

1.4  ZHUIXINGSHAOPING:100ml;

1.5  WEILIANGDIDINGGUAN:5ml、KEDU0.01ml;

1.6  RONGLIANGPING:50ml;

1.7  YIYEGUAN:5ml;

1.8  LIANGTONG:10ml;

1.9  XIERQIU;

1.10  KAISHIWEILIANGZHENGLIUZHUANGZHI(JIANXIATU)、JIAOGUANDENG。

KAISHIWEILIANGZHENGLIUZHUANGZHITU

1-ZHENGLIUPING;2-LENGNINGGUAN;3-CHENGJIEPING;4-HUILIUGUAN-5-LOUDOU;

6-HUOSAI; 7-HUANGJIA;8-SHAOPING

2  SHIJI

2.1  NONGLIUSUAN-GUOYANGHUAQING-SHUIHUNHEYE(2:1:3):ZAI100mlSHUIZHONGHUANMANJIARUNONGLIUSUAN200ml,LENGQUEHOUZAIJIARU30%GUOYANGHUAQING300ml,HUNYUNBEIYONG。CIYECUNFANGYINLIANGCHUKEBAOCUNYIGEYUE。

2.2  混合催化劑:硫酸銅(CuSO4·5H2O)10g,硫酸鉀(A.R)100g,硒粉0.2g,在研體中研細使通過40目篩,混勻后備用;

2.3  40%QINGYANGHUANARONGYE;

2.4  2%PENGSUANRONGYE;

2.5  0.01NYANSUANRONGYE;

2.6  JIAJIHONGYICHUNRONGYE:0.1gJIAJIHONGRONGYU75ml 95%YICHUNZHONG(XIANZAIYANBOZHONGJIAYICHUNYANMO);

2.7  CIJIAJILANYICHUNRONGYE:0.1gCIJIAJILANRONGYU80mL95%YICHUNZHONG。LINYONGSHIJIANGYISHANGLIANGYEAN2:1BILIHUNHEJICHENG。ZAISUANYUCHENGZIHONGSE,ZAIpH5.5SHIRONGYEWUSE,ZAIJIANYUCHENGLVSE。

3  CAOZUOFANGFA

3.1  XIAOHUA

    ANZHAOHANYOU10~30mgCUDANBAIZHIJISUANSHIYANGYONGLIANG,YIBANKECHENGQUSHIYANG0.2~0.3g(W,JINGQUEDAO0.0001g),YONGLAGUANGZHIJUANZHUDAORU50mlKAISHISHAOPINGZHONG,JIAHUNHEZHISHIJI1g,TONGSHIJIARULIUSUAN-GUOYANGHUAQING-SHUIHUNHEYE3~5ml,SHAOJIAZHENYAO,XIEZHIYUDIANLUSHANG,ZAITONGFENGCHUNEIJIAREJINXINGXIAOHUA。KAISHISHIYONGDIWENJIARE,WUSHIPINGZHONGPAOMOCHAOGUOPINGDUDESANFENZHIER,DAIPAOMOJIANSHAOHEYANWUBIANBAIHOUZAIZENGJIAWENDUBAOCHIWEIFEI。XIAOHUADAORONGYECHENGQIANLANLVSEDETOUMINGZHUANGSHI,ZAIJIXUJIAREFEITENG10min,QUCHUDAIXIAOHUAYELENGQUEZHISHIWENHOU,JIASHUI10~20ml,ZAIDAIRONGYEWENDUJIANGDAOSHIWENHOU,GANJINGDIZHUANRU50mlRONGLIANGPINGZHONG,JIASHUIXISHIZHIKEDU,HUNYUNBEIYONG。

3.2  ZHENGLIU

ANZHAOKAISHIWEILIANGZHENGLIUZHUANGZHITUDEANZHUANGJINXINGZHENGLIUHEXISHOU。

ZAISHAOPING8NEIJIASHUI400mlHESHAOLIANGSUIBOLIPIAN,JIA5DIHUNHEZHISHIJI,JIAJIDISUANYESHISHUICHENGZIHONGSE,LIANJIE4,1,2,BINGJIANCHAYOUWULOUQICHU。

LIANGQU30ml 1%PENGSUANRONGYEZHURUZHUIXINGPING3NEI,JIAHUNHEZHISHIJI2DI,SHILENGNINGGUANXIADUANCHARUYEMIANXIA1cmCHU。LIANGQUXISHIDEXIAOHUAYE5ml,YOULOUDOU5ZHURUPING1NEI,ZAIJIARU10ml,CHONGXILOUDOU5。LIANGQU40%QINGYANGHUANARONGYE1ml,TIQIHUOSAIZHURUPING1NEI,LIJIYONGSHUI2~5mlCHONGXILOUDOU5,XUANJINHUOSAI。ZHESHIPING1NEIRONGYEZONGTIJIBUYAOCHAOGUOQIRONGLIANGDEYIBAN。

DAKAIHUANGJIA7,GUANBIHUOSAI6,JIARESHAOPING8,DAIPING1NEIRONGYEKAISHIFEITENGSHIKAISHIJISHI,JING2minJIANGDIPING3,SHILENGNINGGUANXIADUANLUCHUYEMIAN,ZAIZHENGLIU1minHOU,YONGSHUICHONGXIGUANXIADUAN。

ZHENGLIUJIESHUHOU,QIAJINHUANGJIA7,DUANJUEZHENGQI,SHIPING1NEIRONGYEQUANBUXIRUGUAN4ZHONG,FANGSONGHUANGJIA7,CONGLOUDOU5JIARUZHENGLIUSHUI40~50ml,ZAITONGZHENGQIJIAREHEHUILIU,JIANGPING1XIDIYICIBEIYONG。

3.3  DIDING

YONG0.01NYANSUANRONGYEDIDINGPING3ZHONGDEXISHOUYE,DIZHIRONGYECHUXIANQIANZIHONGSEWEIZHI。

WEIXIAOCHUSHIJIWUCHA,CHUBUJIASHIYANGWAI,ANZHAOSHANGSHUCAOZUOFANGFA,ZUOYICIKONGBAISHIYAN。

4  JIEGUOJISUAN

CUDANBAIZHIDEGANJIHANLIANGANXIALIEGONGSHIJISUAN:

粗蛋白質(干基%)

SHIZHONG:V――ZHENGLIUSHIQUYONGXISHIDEXIAOHUAYETIJI,ml;

     V1――實驗用去的鹽酸溶液體積,ml;

         V0――空白試驗用去的鹽酸溶液體積,ml;

         N――YANSUANRONGYEDEDANGLIANGNONGDU,N;

         14――MEIHAOKEDANGLIANGYANSUANXIANGDANGYUDANDEKESHU;

         P――DANBAIZHIHUANSUANXISHU(XIAOMAI5.7,QITAGUWUJIDOULEI6.25);

         W――SHIYANGZHONGLIANG,mg;

         M――SHIYANGSHUIFENBAIFENLV,%。

SHUANGSHIYANJIEGUOYUNXUCHA:CUDANBAIZHIHANLIANGZAI15.0%YIXIASHI,BUCHAOGUO0.2%;CUDANBAIZHIHANLIANGZAI15.1%YISHANGSHI,BUCHAOGUO0.4%。QIUQIPINGJUNSHU,JIWEICEDINGJIEGUO,CEDINGJIEGUOQUXIAOSHUDIANHOUDIYIWEI。

ZHU:①XIAOHUAGUOCHENGZHONG,RUOLIUSUANSUNSHIGUODUOSHI,KEZHUOLIANGBUJIALIUSUAN,WUSHIPINGNEIGANHE。

    ②XIAOHUAYEJIARUXISHIHOU,YINGJISHIJINXINGZHENGLIU,FOUZEYINGBAOCUNXIAOHUAYE,LINYONGSHIJIASHUIXISHI。

③JIARUZHENGLIUPING1NEIDEJIANYE,BIXUGUOLIANG。

④YEKESHIYONGYOU0.2%JIAJIHONGYICHUNRONGYE1FENHE0.2%XIUJIAFENLVYICHUNRONGYE5FENPEIZHIDEHUNHEZHISHIJI(LINYONGSHIHUNHE),ZHONGDIANWEIHUIHONGSE。

 

 

 

 

FULUA

DADOUSHUIRONGXINGDANBAIZHICEDINGFA

(BUCHONGJIAN)

 

A.1  YIQIHEYONGJU

1.1  FENSUIJI;

1.2  MOKOUDAISAIZHUIXINGPING:500ml;

1.3  ZHENDANGQI:GUOJIXING;

1.4  RONGLIANGPING:250ml;

1.5  離心機,附50ml離心管;

1.6  BOLILOUDOU;

1.7  ZHUIXINGPING、YIYEGUAN;

1.8  QITAYIQIHEYONGJUYUBENBIAOZHUNDI1ZHANGTONG。

A.2  SHIJI

SUOYONGSHIJIYUBENBIAOZHUNDI2ZHANGTONG。

A.3  CAOZUOFANGFA

A.3.1  SHIYANGZHIBEI:JIANGDADOUFENSUISHI90%YISHANGTONGGUO60MUSHAI。

A.3.2  TIQU:CHENGQUFENSUISHIYANG5g(W,JINGQUEZHI0.01g)YUMOKOUDAISAIZHUIXINGPINGZHONG,JIASHUI200ml,YAOHUNSHIJUNYUNFENSAN,RANHOUZAI25~30℃WENDUXIAZHENDANG2h,QUCHUHOUJIANGHUNHEYEZHUANYIZHI250mlRONGLIANGPINGZHONG,YONGSHUIXISHIZHIKEDU,HUNYUNHOUJINGZHI1~2min,JIANGSHANGCENGQINGYEDAORULIXINGUANZHONG,ZAIMEIFENZHONGZHUANSHU1500ZHUANDELIXINJIZHONGLIXIN10min,ZAIJIANGLIXINYEYONGKUAISULVZHIHUOBOLIXIANWEIGUOLV,SHOUJIQINGXILVYEYUZHUIXINGSHAOPINGZHONG,JIWEIYANGPINSHUIRONGXINGDANBAIZHICEDINGYE。

A.3.3  DINGDAN:XIQUCEDINGYE10mlYU50mlHUO100mlKAISHIDINGDANPINGZHONG,ANBENBIAOZHUN3.1、3.2、3.3BUZOUJINXINGXIAOHUA、ZHENGLIU、DIDING。JIXIADIDINGYANGPINYEJIKONGBAIYEXIAOHAOYANSUANDESHULIANG。

A.4  JIEGUOJISUAN

SHUIRONGXINGDANBAIZHIHANLIANGANXIALIEGONGSHIJISUAN:

水溶性蛋白質(干基%)=

式中:V1――滴定5ml樣品液消耗鹽酸體積,ml;

      V0――滴定5ml空白液消耗鹽酸體積,ml;

      N――YANSUANRONGYEDEDANGLIANGNONGDU,N;

     0.014――MEIHAOKEDANGLIANGDANDEKESHU;

     6.25――DADOUHANDANLIANGHUANSUANCHENGDANBAIZHIXISHU;

     10――XISHOUTIQUYEJINXINGXIAOHUADETIJI,ml;

    250――ZONGTIQUYEDETIJI,ml;

      5――XISHOUXIAOHUAYEJINXINGZHENGLIUDETIJI,ml

     50――ZONGXIAOHUAYEDETIJI,ml;

    W――SHIYANGZHONGLIANG,g;

    M――SHIYANGSHUIFENBAIFENLV,%。

SHUANGSHIYANJIEGUOYUNXUCHABUCHAOGUO0.4%,QIUQIPINGJUNSHU,JIWEICEDINGJIEGUO。CEDINGJIEGUOQUXIAOSHUDIANHOUDIYIWEI。

ZHU:①DADOUDANKERONGJIEZHISHU(%)DEJISUANFANGFASHI:YISUOCEDEDEDADOUSHUIRONGXINGDANBAIZHI(%)CHUYIDADOUZONGDANBAIZHI(%),ZAICHENGYI100JIDE。

②BENFANGFACAIYONGYANGPINZHIBEIFANGFAJITIQUFANGFASHICEDINGJIEGUOJIAOWEIWENDING,DANRUYINFENSUIYANGPINXIDUDABUDAOYAOQIU,JIKECAIYONGCHENGQUSHIYANG5.00gYUYANBOZHONG,JIA5mlSHUI,YONGSHOUYANMO10min,JIANGHUNHEWUYONG200mlSHUIZHUANYIZHI250mlMOKOUPINGZHONG,ZAIZAIZHENDANGQISHANGZHENDANG30min,RANHOUTONGBENBIAOZHUNDE3.2JINXINGDINGRONG、LIXIN、GUOLV、DINGDAN。

 

 

 

 


FUJIASHUOMING:

BENBIAOZHUNYOUZHONGHUARENMINGONGHEGUOSHANGYEBUTICHU。

BENBIAOZHUNYOUSHANGYEBULIANGSHICHUYUNJUFUZEQICAO。

BENBIAOZHUNZHUYAOQICAORENGAOXIUWU、YANGHAORAN、WUYANXIA、LVGUIFEN。

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